Investigative

Cell Biology

SERVICES

Services

Angiogenesis assay

High-content imaging based angiogenesis assay in 384-well format using donor-specific endothelial colony forming cells, HUVECs, or customer provided endothelial cells.

In Vitro ADME-Tox

Extended range of non-clinical services to the customers requiring reliable data for lead optimization prior to in vivo testing using our cost-effective high-throughput in vitro Adsorption, Distribution, Metabolism, and Excretion testing.

LAL Endotoxin testing

Endotoxin testing using quantitative Kinetic Chromogenic method based on Limulus Amebocyte Lysate (LAL) reagent. Our test detects endotoxin in the range of 0.005 to 50 endotoxin units per milliliter.

cellular nitric oxide assay

High content imaging based in vitro nitric ooxide detection using a novel highly specific nitric oxide probe.

Products

VecStem

The VecStem Endothelial Cell Culture Media from Creative Scientist, Inc. is specially formulated and optimized for robust endothelial cell growth. The complete VecStem media contains 2% human serum, recombinant growth factors, and vitamins.

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Core technology

CORE TECHNOLOGY

Population-wide assessment of human health is challenging because of intrinsic genetic diversity complicated by age and other non-genetic factors, such as exposure to environmental stressors. To assess human responses to drugs and toxicants, we develop functional in vitro cell-based assays using highly proliferative primary human cells non-invasively isolated from human tissues.

To study the diversity of responses to drugs and toxicants we compile libraries of Highly Proliferative Primary Cells by growing them in culture and cryopreserving for many years, thus enabling to conduct hundreds of assays from a single donor.

Endothelial Colony Forming Cells

PROJECTS

CELL BASED TOXICOLOGY

Donor-Derived Progenitor Cells for Population-Wide Toxicity Testing

Environmental toxicants affect people differently. A substance harmless to one person can be toxic to another because of genetics, or previous experiences, or both. To identify such vulnerable people we are developing special assay platform utilizing donor-derived progenitor cells.

RADIATION EXPOSURE

We develop an assay platform utilizing donor-specific endothelial progenitor cells as a proxy to test individual sensitivity to ionizing radiation (IR). IR is known to cause long-term complications. In our method, individual IR sensitivity is correlated to an in vitro response to radiation of endothelial progenitor cells, also called endothelial colony forming cells (ECFCs), which critically contribute to the healing of injured tissues. ECFC recruitment, proliferation, and differentiation have been proposed to be a key to tissue recovery after radiation-induced damage. Our assay platform will help to test individual susceptibility to radiation-mediated damage and enable the discovery of novel radioprotectors.