Investigative Cell Biology

Accelerate R&D
Improve Data Quality
Enhance Relevance

Our Services: Scalable Multi-Well Assays

Vascular Capillary Networks

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Spheroids and Tumoroids

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Barrier Tissues Assays

In Vitro ADME-Tox

 (910) 621-5451‬ •  csi@cscientist.com

Cell-Based Assays

High-content cell imaging
Cell viability, cytotoxicity & signaling
2D & 3D angiogenesis in 384-well plates
96-well tumor spheroids for drug testing
96-well TEER & transport testing in barrier tissues
Multi-color FACS analysis

Molecular Biology

Chromatography for protein purification
Electrophoresis & Western for protein detection
Antigen-antibody interaction
DLS/SEC/MALS for particles & aggregates
AAV & lentivirus production
Analytical Ultracentrifugation

Services

VecStem™: Endothelial Cell Growth Media

Products

The VecStem Endothelial Cell Culture Media from Creative Scientist, Inc. is specially formulated and optimized for robust endothelial cell growth. The complete VecStem media contains 2% human serum, recombinant growth factors, and vitamins.

Core technology

Core Technology

Population-wide assessment of human health

This is a challenging topic because of intrinsic genetic diversity complicated by age and other non-genetic factors, such as exposure to environmental stressors. To assess human responses to drugs and toxicants, we develop functional in vitro cell-based assays using highly primary human cells isolated from peripheral blood.

To study the diversity of responses to drugs and toxicants we build a collection of human progenitor cells. These cells can be used in multiple assays for population studies in a dish.

Endothelial Colony Forming Cells

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PROJECTS

Case Study 1: Cell-Based Toxicology

Donor-Derived Progenitor Cells for Population-Wide Toxicity Testing

Environmental toxicants affect people differently. A substance harmless to one person can be toxic to another because of genetics, or previous experiences, or both. To identify such vulnerable people we are developing special assay platform utilizing donor-derived progenitor cells.

Case Study 2: Radiation Exposure

Donor-Derived Endothelial Progenitor Cells Are Sensitive to Radiation

We have developed an assay platform utilizing donor-specific endothelial progenitor cells as a proxy to test individual sensitivity to ionizing radiation (IR). IR is known to cause long-term complications. In our method, individual IR sensitivity is correlated to an in vitro response to radiation of endothelial progenitor cells, also called endothelial colony forming cells (ECFCs), which critically contribute to the healing of injured tissues. ECFC recruitment, proliferation, and differentiation have been proposed to be a key to tissue recovery after radiation-induced damage. Our assay platform helps to analyze individual susceptibility to radiation-mediated damage and enables the discovery of novel radioprotectors.

Team